A study of the properties and methods of analysis of high molecular weight N-nitrosamines

Abstract

Various high molecular weight dialkylnitrosamines were prepared including, for the first time, methyl-n-octadecylnitrosamine and di-n-dodecylnitrosamine. The infrared, ultraviolet and mass spectra of a selection of these compounds were recorded and studied. Gel permeation chromatography was used for the isolation of individual nitrosamines in standard nitrosamine mixtures, while ion-exchange chromatography effected complete clean-up of amine-contaminated nitrosamine solutions. Thin-layer and gas-liquid chromatographic methods were developed for the detection, separation and analysis of nanogram quantities of these lipophilic nitrosamines. In addition the above chromatographic systems were used for the analysis of distillates of spiked wheat flour samples. High recoveries of dicyclopentylnitrosamine, di-n-heptylnitrosamine and di-n-octylnitrosamine, from the spiked wheat flour samples, were achieved using a specially developed freeze-drying/vacuum distillation technique, the distillates obtained being relatively free from major contaminants.,KMBT_363,Adobe Acrobat 9.54 Paper Capture Plug-in