The biotransformation of phenolic pollutants using polyphenol oxidase

dc.contributor.author	Boshoff, Aileen
dc.date.accessioned	2026-03-04T13:57:29Z
dc.date.issued	2002
dc.description.abstract	The potential of using mushroom polyphenol oxidase (EC 1.14.18.1) as a biocatalyst for the biotransformation of phenols to produce catechols in an aqueous medium was investigated. Polyphenol oxidase is characterised by two distinct reactions i.e., the ortho-hydroxylation of phenols to catechols (cresolase activity) and the subsequent oxidation of catechols to orthoquinones (catecholase activity). In order to facilitate the development of a process to produce catechols, the accumulation of catechol as a true intermediate product released in the reaction system needed to be investigated, as its release had been disputed due to the oxidation of catechols to o-quinones. Using LC-MS, catechol products were successfully identified as true intermediate products formed during biocatalytic reactions in water.
dc.description.degree	Doctoral thesis
dc.description.degree	PhD
dc.format.extent	227 pages
dc.format.mimetype	application/pdf
dc.identifier.other	http://hdl.handle.net/10962/d1004035
dc.identifier.uri	https://researchrepository.ru.ac.za/handle/123456789/7061
dc.language	English
dc.publisher	Rhodes University, Faculty of Science, Department of Biochemistry, Microbiology and Biotechnology
dc.rights	Boshoff, Aileen
dc.subject	Polyphenol oxidase
dc.subject	Sewage -- Purification
dc.title	The biotransformation of phenolic pollutants using polyphenol oxidase
dc.type	Academic thesis

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